Measuring soluble analytes is an attractive way to monitor treatment effects, and potentially also local tumor events, because secreted proteins or molecules shed through exosomes can be repeatedly sampled in the periphery. The Proseek Olink Proteomics platform operating on a Fluidigm Biomark HD microfluidic PCR system is a quantitative and reproducible assay to measure levels of molecules related to inflammation. The assay can be applied to peripheral serum or plasma, as well as tissue culture supernatants, and requires only 1 microliter of sample.

Mount Sinai-CIMAC is the first center in the US that has been trained and certified to perform these assays in house. The Proximity Extension Assay (PEA) technology that underlies these assays relies on dual antibody recognition and incorporates several innovative QC steps (including three different external controls and two internal controls) to evaluate each step of the assay protocol, resulting in an extremely robust assay. Critically, in addition to profiling cytokines, chemokines and growth factors, this platform also allows detection of circulating immune co-stimulatory and inhibitory molecules, and other relevant immune-oncology markers, in a 92-plex format. Plasma or serum will be collected and frozen and used for Olink multiplex immunoassay at Mount Sinai-CIMAC. Markers included in the panel are described in Table 1

Adenosine Deaminase (ADA)
Adhesion G-protein coupled receptor G1 (ADGRG1)
Angiopoietin-1 (ANG-1)
Angiopoietin-1 receptor (TIE2)
Angiopoietin-2 (ANGPT2)
Arginase-1 (ARG1)
C-C motif chemokine 17 (CCL17)
C-C motif chemokine 19 (CCL19)
C-C motif chemokine 20 (CCL20)
C-C motif chemokine 23 (CCL23)
C-C motif chemokine 3 (CCL3)
C-C motif chemokine 4 (CCL4 )
C-X-C motif chemokine 1 (CXCL1)
C-X-C motif chemokine 10 (CXCL10)
C-X-C motif chemokine 11 (CXCL11)
C-X-C motif chemokine 13 (CXCL13)
C-X-C motif chemokine 5 (CXCL5)
C-X-C motif chemokine 9 (CXCL9)
Carbonic anhydrase IX (CAIX)
Caspase-8 (CASP-8)
CD27 antigen (CD27)
CD40 ligand (CD40-L)
CD40L receptor (CD40)
CD70 antigen (CD70)
CD83 antigen (CD83)
Cytotoxic and regulatory T-cell molecule (CRTAM)
Decorin (DCN)
Fas antigen ligand (FasL)
Fibroblast growth factor 2 (FGF2)
Fractalkine (CX3CL1 )
Galectin-1 (Gal-1)
Galectin-9 (Gal-9)
Granzyme A (GZMA)
Granzyme B (GZMB)
Granzyme H (GZMH)
Heme oxygenase 1 (HO-1)
Hepatocyte growth factor (HGF)
ICOS ligand (ICOSLG)
Interferon beta (IFN-beta)
Interferon gamma (IFN-gamma)
Interleukin-1 alpha (IL-1 alpha)
Interleukin-10 (IL10)
Interleukin-12 (IL-12)
Interleukin-12 receptor subunit beta-1 (IL12RB1)
Interleukin-13 (IL-13)
Interleukin-18 (IL-18)
Interleukin-2 (IL-2)
Interleukin-21 (IL-21)
Interleukin-33 (IL-33)
Interleukin-35 (IL-35)
Interleukin-4 (IL-4)
Interleukin-5 (IL5)
Interleukin-6 (IL6)
Interleukin-7 (IL-7)
Interleukin-8 (IL-8)
Latency-associated peptide transforming growth factor beta-1 (LAP TGF-beta-1)
Lysosome-associated membrane glycoprotein 3 (LAMP3)
Macrophage colony-stimulating factor 1 (CSF-1)
Matrix metalloproteinase-12 (MMP-12)
Matrix metalloproteinase-7 (MMP-7)
MHC class I polypeptide-related sequence A/B (MIC-A/B)
Monocyte chemotactic protein 1 (MCP-1)
Monocyte chemotactic protein 2 (MCP-2)
Monocyte chemotactic protein 3 (MCP-3)
Monocyte chemotactic protein 4 (MCP-4)
Natural cytotoxicity triggering receptor 1 (NCR1)
Natural killer cell receptor 2B4 (CD244)
Natural killer cells antigen CD94 (KLRD1)
Nitric oxide synthase, endothelial (NOS3)
Placenta growth factor (PGF)
Platelet-derived growth factor subunit B (PDGF subunit B)
Pleiotrophin (PTN)
Pro-epidermal growth factor (EGF)
Programmed cell death 1 ligand 1 (PD-L1)
Programmed cell death 1 ligand 2 (PD-L2)
Programmed cell death protein 1 (PDCD1)
Stromal cell-derived factor 1 (CXCL12)
T-cell surface glycoprotein CD4 (CD4)
T-cell surface glycoprotein CD5 (CD5)
T-cell surface glycoprotein CD8 alpha chain (CD8A)
T-cell-specific surface glycoprotein CD28 (CD28)
TNF-related apoptosis-inducing ligand (TRAIL)
Tumor necrosis factor (Ligand) superfamily, member 12 (TWEAK)
Tumor necrosis factor (TNF)
Tumor necrosis factor ligand superfamily member 14 (TNFSF14 )
Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A)
Tumor necrosis factor receptor superfamily member 21 (TNFRSF21)
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4 )
Tumor necrosis factor receptor superfamily member 9 (TNFRSF9)
Vascular endothelial growth factor A (VEGF-A)
Vascular endothelial growth factor C (VEGFC)
Vascular endothelial growth factor receptor 2 (VEGFR-2)

Table 1: Olink Immuno-Oncology panel

Olink is available at Mount Sinai CIMAC only.